Are you sure that a polynomial is an appropriate calibration curve here? You
don't give enough points to assess the fit, but I am much more familiar with
OD calibration curves that are either log-linear or a 4- or 5-parameter
logistic equation.

Jerry

"PAUL GRAZIDE" wrote:

I am setting up a chemistry calibration curve using data below

OD reading Concentration
..020 0
..045 10
..245 40 etc

Plotting in XY scatter chart and get trendline using polynomial function.
How can I determine the concentration of an unkown solution ? I want to use
trendline data to do this.